Political culture means structures, values, attitudes, and feelings towards politics and political behavior in society. Given the importance of political culture, the following research uses the Laclau and Mouffe discourse analysis method to study the political culture of Iranian religious intellectuals after the Islamic Revolution. The statistical population includes 20 texts from five religious intellectuals. The findings show that the central signifier of the discourse of these intellectuals was “critique of political power,” which is articulated with floating signifiers including “freedom,” “human rights,” “dialogue and negation of violence,” “civil society,” and “critique of tradition,” which find meaning within the framework of the central signifier. This discourse is a reflection of a participatory political culture that is based on adherence to modern values such as human rights, pluralism, and critical rationality. Religious intellectuals, emphasizing the compatibility of religion and modernity, seek to redefine the relationship between tradition and modernity in the Iranian political space. The results of the research indicate that this discourse has played an effective role in shaping Iran's political and social developments after the revolution and examines the challenges of advancing political development with a critical and reformist perspective.